Humorous Phases of Funny Faces
Humorous Phases of Funny Faces is a 1906 short silent animated cartoon directed by James Stuart Blackton and generally regarded by film historians as the first animated film recorded on standard picture film.
Stopmotion as well as cutout animation are used, just as Edwin Porter moved his letters in How Jones Lost His Roll, and The Whole Dam Family and the Dam Dog. However, there is a very short section of the film where things are made to appear to move by altering the drawings themselves from frame to frame.
Source: Wikipedia